Sanitary survey of brucellosis in goats from productive systems of the northeast of chaco: first regional microbiological record of the isolation of B. Melitensis from goat abortions
Keywords:
goats, Brucella melitensis, isolation, Argentina, bacteriologyAbstract
The study was conducted in the village of Miraflores, county of General Güemes, Chaco, Argentina. Seven farms were selected due to their high prevalence to caprine brucellosis, previously determined by Buffered Plate Antigen (BPA) and Fluorescence Polarization immunoassay (FPA) serological tests. In each farm, blood samples without anticoagulants were collected from all the animals from the jugular vein. An additional sampling in females who had aborted in the past 15 days was carried out, consisting in vaginal swabs, blood and milk from the udder. From 98 blood samples, 20 milk samples and 7 vaginal swabs, 8 positive cultures, three from vaginal swabs and five from buffy coat cultures were obtained from 3 of the seven farms. The cultures obtained were submitted to traditional typification. Results showed that the isolated strains in Miraflores corresponded to Brucella melitensis biovar 1, being this in line with previous reports in the region and the country. The isolation of B. melitensis and its relationship with abortions in goats, confirms the high impact of the disease in goat production and reinforces the importance of this disease, not only in goats but also in rural families.
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References
-Alton, G.G. (1990) Brucella melitensis. En: Animal Brucellosis, Ed. por Nielsen y Duncan. CRC Press, Boca Ratón, Florida, USA.
-Alton, G.G.; Jones, L.M.; Angus, R.D.; Verger, J.M. (1988) Techniques for the brucellosis laboratory. 1st edition. Institut National de la Recherche Agronomique. París.
-Coelho, A., Díez, J. G., & Coelho, A. C. (2014) Brucelosis en pequeños rumiantes: etiológia, epidemiología, sintomatología, diagnóstico, prevención y control. REDVET. Revista Electrónica de Veterinaria, 15(5),1-31. https://www.redalyc.org/articulo.oa?id=63633881002
-Corbel MJ, Thomas EL. (1985) Use of phage for the identification of Brucella canis and Brucella ovis cultures. Res Vet Sci.; 35: 35-40).
-Corbel MJ, Banai M. (2005) Genus Brucella, Meyer and Shaw 1920, 173AL En: Brenner DJ, Krieg NR, Staley JT, Garrity GM, editors. Bergey´s Manual of Systematic Bacteriology. New York: Springer, vol. 2, p. 370-867
-Corbel MJ. (2006) Brucellosis in humans and animals. FAO/OIE/WHO, WHO/CDS/EPR/2006.7
- Dawood, A. S., Elrashedy, A., Nayel, M., Salama, A., Guo, A., Zhao, G., Algharib, S. A., Zaghawa, A., Zubair, M., Elsify, A., Mousa, W., & Luo, W. (2023). Brucellae as resilient intracellular pathogens: epidemiology, host-pathogen interaction, recent genomics and proteomics approaches, and future perspectives. Frontiers in veterinary science, 10, 1255239. https://doi.org/10.3389/fvets.2023.1255239
-De Miguel, M. J., Marín, C. M., Muñoz, P. M., Dieste, L., Grilló, M. J., & Blasco, J. M. (2011). Development of a selective culture medium for primary isolation of the main Brucella species. Journal of clinical microbiology, 49(4), 1458–1463. https://doi.org/10.1128/JCM.02301-10
-Díaz Aparicio, E. (2013) Epidemiología de la brucelosis causada por Brucella melitensis, Brucella suis y Brucella abortus en animales domésticos. Rev.sci.tech Off. int. Epiz. 32 (1), 43-51.
-El-Diasty, M., Wareth, G., Melzer, F., Mustafa, S., Sprague, L. D., & Neubauer, H. (2018). Isolation of Brucella abortus and Brucella melitensis from Seronegative Cows is a Serious Impediment in Brucellosis Control. Veterinary sciences, 5(1), 28. https://doi.org/10.3390/vetsci5010028
-Franc, K. A., Krecek, R. C., Häsler, B. N., & Arenas-Gamboa, A. M. (2018). Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action. BMC public health, 18(1), 125. https://doi.org/10.1186/s12889-017-5016-y
-Gaido AB, Micheloud JF, Torioni de Echaide S, Neder V, Campero CM, Suárez VH, Neumann RD, Aguirre DH (2015). Primer reporte de aborto ovino por Brucella melitensis en una majada mixta con caprinos de la provincia de Salta, Argentina. 9no Seminario de la Fundación "Charles Louis Davis" en Salta, Argentina. (3) (PDF) PRIMER REPORTE DE ABORTO OVINO POR Brucella melitensis EN UNA MAJADA MIXTA CON CAPRINOS DE LA PROVINCIA DE SALTA, ARGENTINA. (researchgate.net)
Garin-Bastuji, B., Mick, V., Le Carrou, G., Allix, S., Perrett, L. L., Dawson, C. E., Groussaud, P., Stubberfield, E. J., Koylass, M., & Whatmore, A. M. (2014). Examination of taxonomic uncertainties surrounding Brucella abortus bv. 7 by phenotypic and molecular approaches. Applied and environmental microbiology, 80(5), 1570–1579. https://doi.org/10.1128/AEM.03755-13
-Herrera, E., Rivera, A., Palomares, E.G. et al. Isolation of Brucella melitensis from a RB51-vaccinated seronegative goat. Trop Anim Health Prod 43, 1069–1070 (2011). https://doi.org/10.1007/s11250-011-9822-4
-Lucero, N. E.; Ayala, S.M.; Escobar, G.I.; Jacob, N.R. (2008) Brucella isolated in humans and animals in Latin America from 1968 to 2006. Epidemiol. Infect. 136, 496-503. https://doi.org/10.1017/S0950268807008795
-Martínez Herrera, D.I, Abeledo, María A, Lara Gutiérrez, A, Peniche Cardeña, A, Robledo Salinas, M.L, Pulido Camarillo, E, Rosas Sastre, T.J, & Flores Castro, R. (2009). Evaluación de métodos de cultivo para el aislamiento primario de Brucella melitensis a partir de leche de cabras. Revista de Salud Animal, 31(3), 164-169. Recuperado en 28 de junio de 2022, de http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000300005&lng=es&tlng=es
-Martínez, D. E., Cipolini, M. F., Storani, C. A., Russo, A. M., & Martínez, E. I. (2018). Brucelosis: prevalencia y factores de riesgo asociados en bovinos, bubalinos, caprinos y ovinos de Formosa, Argentina. Revista Veterinaria, 29(1), 40. https://doi.org/10.30972/vet.2912789
-Megid, Jane & Mathias, Luis & Robles, Carlos. (2010). Clinical Manifestations of Brucellosis in Domestic Animals and Humans. The Open Veterinary Science Journal. 4. 10.2174/1874318801004010119
-Mena-Bueno, S., Poveda-Urkixo, I., Irazoki, O., Palacios, L., Cava, F., Zabalza-Baranguá, A., & Grilló, M. J. (2022). Brucella melitensis Wzm/Wzt System: Changes in the Bacterial Envelope Lead to Improved Rev1Δwzm Vaccine Properties. Frontiers in Microbiology, 13. https://doi.org/10.1128/spectrum.01759-22
-Monzón N.M., Cipolini M.F., Martínez D.E., Espansandin A.G. , Lozina J., Arzú R., Yulán V., Robles C. (2021) Seroprevalencia de brucelosis caprina en el impenetrable chaqueño: resultados preliminares. AAVLD XXIII Reunión Científico Técnica: 17, 18 y 19 de noviembre de 2021. 1a ed. - Balcarce: Asoc. Argentina de Veterinarios de Laboratorios de Diagnóstico, 2021. Libro digital, PDF. ISBN 978-987-21667-3-1
-Nicola A.M.; Elena S.; Franco, C. (2019) Manual de diagnóstico serológico de la brucelosis bovina SENASA. Laboratorio de Referencia de OIE para brucelosis. Argentina. brucelosis_manual_2019_prot.pdf (argentina.gob.ar)
-Nielsen, K., Gall, D., Smith, P., Bermudez, R., Moreno, F., Renteria, T., & Halbert, G. (2005a). Evaluation of serological tests for detection of caprine antibody to Brucella melitensis. Small Ruminant Research, 56(1-3), 253-258. https://doi.org/10.1016/j.smallrumres.2004.06.006
-Nielsen, K., Smith, P., Yu, W., Nicoletti, P., Elzer, P., Robles, C., Bermudez, R., Renteria, T., Moreno, F. S., Ruiz, A., Massengill, C., Muenks, Q., Jurgersen, G., Tollersrud, T., Samartino, L., Conde, S., Forbes, L., Gall, D., Perez, B., Rojas, X., … Minas, A. (2005b). Towards single screening tests for brucellosis. Revue scientifique et technique (International Office of Epizootics), 24(3), 1027–1037. https://doi.org/10.20506/rst.23.3.1532
-Resolución Nº372-E (2017). Servicio Nacional de Sanidad y Calidad Agroalimentario, Buenos Aires, Argentina, 14 de junio. Resolución-372-2017-SENASA - Servicio Nacional de Sanidad y Calidad Agroalimentaria (argentina.gob.ar)
-Resolución Nº857-E. (2017) Servicio Nacional de Sanidad y Calidad Agroalimentario, Buenos Aires, Argentina, 22 de diciembre. D:livecycletmppdfg-DMZGDEMSAPPB015_35fe06-8b4885-9399eb-c06991-fa522e-ab16c2File.html (senasa.gob.ar)
-Robles, C.; Gaido, A.; Späth, E.; Torioni de Echaide, S.; Vanzini, V.; Zielinsky, G.; Aguirre, D.; Samartino, L.; Rossanigo, C. (2014) Brucelosis caprina en la Argentina. Ediciones INTA, 1era edición. 29 pag. ISBN 978-987-521-557-3. http://hdl.handle.net/20.500.12123/7597
-Rossetti, C. A., Arenas-Gamboa, A. M., & Maurizio, E. (2017). Caprine brucellosis: A historically neglected disease with significant impact on public health. PLoS neglected tropical diseases, 11(8), e0005692. https://doi.org/10.1371/journal.pntd.0005692
-Rossetti, C. A., Maurizio, E., & Rossi, U. A. (2022). Comparative Review of Brucellosis in Small Domestic Ruminants. Frontiers in veterinary science, 9, 887671. https://doi.org/10.3389/fvets.2022.887671
-Russo, A.M.; Mancebo, O.; Monzón, C.; Gait, J.; Casco R.; Torioni, S. (2016) Epidemiología de la brucelosis caprina y ovina en la provincia de Formosa, Argentina. Revista Argentina de Microbiología. 48 (2),147-153. http://dx.doi.org/10.1016/j.ram.2015.10.005
-Saavedra, Maria & Fernandes, Conceição & Queiroga, Cristina. (2019). LABORATORY DIAGNOSIS OF BRUCELLOSIS. In: Brucellosis in Goats and Sheep ISBN: 978-1-53614-962-3Editors: J. C. Caetano Simões et al. Nova Science Publishers, Inc
-Tekle, M., Legesse, M., Edao, B. M., Ameni, G., & Mamo, G. (2019). Isolation and identification of Brucella melitensis using bacteriological and molecular tools from aborted goats in the Afar region of north-eastern Ethiopia. BMC microbiology, 19(1), 108. https://doi.org/10.1186/s12866-019-1474-y
-World Organization for Animal Health (OIE). (2022) Brucellosis (Brucella abortus, B. melitensis and B. suis) (Infection with B. abortus, B. melitensis and B. suis). OIE Terrestrial Manual. Brucellosis (woah.org)
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