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Molecular detection of Ehrlichia canis in dogs from different locations of the province of Córdoba, Argentina

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Abstract

Tick-borne agents are an emergent problem which can cause severe diseases and/or sub-clinic infections in dogs. Ticks play an important role as reservoir of these microorganisms which can infect other dogs and even humans. Canine monocytic ehrlichiosis is one of the most frequent tick-borne diseases. It is transmitted to the dog through an infected tick (Rhipicephalus sanguineus) bite, and its spreads through the blood and the lymphatic system to liver, spleen and lymph nodes. This disease has been diagnosed in dogs from different areas of the country; however there are not records about its molecular diagnosis in Córdoba province. The aim of this work was to determine the presence of Ehrlichia canis in dogs with symptoms compatible with this infection; for this purpose, PCR were performed on dog’s blood from the city of Río Cuarto and the towns of Alta Gracia and Adelia María, where 8 positive cases were found.

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References

Allison, R.W., Little, S.E. (2013). Diagnosis of rickettsial diseases in dogs and cats. Veterinary Clinical Pathology, 42 (2):127-144.

Aubert, S.R., Rossanigo, C.E., Crosa, P., Serrano, D., De Salvo, M.N., Cicuttin, G.L. (2016). Un caso de ehrlichiosis en un perro de la provincia de San Luis. Conferencia: XXI Reunión Científico Técnica de la Asociación Argentina de Veterinarios de Laboratorios de Diagnóstico (AAVLD). Memorias de resúmenes.

Baneth, G. (2014). Tick-borne infections of animals and humans: a common ground. International Journal for Parasitology, 44 (9):591-596.

Baneth, G., Harrus, S., Ohnona, F.S., Schlesinger, Y. (2009). Longitudinal quantification of Ehrlichia canis in experimental infection with comparison to natural infection. Veteterinary Microbiology, 136 (3-4):321-325.

Baneth, G., Samish, M., Alekseev, Y., Aroch, I., Shkap, V. (2001). Transmission of Hepatozoon canis to dogs by naturally fed or percutaneously injected Rhipicephalus sanguineus ticks. Journal of Parasitology, 87, 606-611.

Borrás, P., Sánchez, J., Guillemi, E., De La Fourniere, S., Abadía, M., Farber, M., Santini, M.S. (2019). Detección de bacterias de los géneros Ehrlichia, Anaplasma y Rickettsia en garrapatas Rhipicephalus sanguineus sl en Pergamino Argentina. Revista Argentina de Salud Pública, 10(41):8-13.

Bremer, W.G., Schaefer, J.J., Wagner, E.R., Ewing, S.A., Rikihisa, Y., Needham, G.R., Jittapalapong, S., Moore, D.L., Stich, R.W. (2005). Transstadial and intrastadial experimental transmission of

Ehrlichia canis by male Rhipicephalus sanguineus. Veterinary Parasitology, 131(1/2):95-105.

Castro, M.B., Machado, R.Z., Aquino, L.P.T., Alessi, A.C., Costa, M.T. (2004). Experimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findings. Veterinary Parasitology, 119:73-86.

Çetinkaya, H., Matur, E., Akyazi, İ., Ekiz, E.E., Aydin, L., Toparlak, M. (2016). Serological and molecular investigation of Ehrlichia spp. and Anaplasma spp. in ticks and blood of dogs, in the Thrace Region of Turkey. Ticks and Tick Borne Diseases, 7(5):706-714.

Cicuttin, G.L., De Salvo, M.N., Gury Dohmen, F.E. (2016). Molecular characterization of Ehrlichia canis infecting dogs, Buenos Aires. Ticks and Tick Borne Diseases, 7(5):954-957.

Cicuttin, G.L., De Salvo, M.N., Nava, S. (2017). Two novel Ehrlichia strains detected in Amblyomma tigrinum ticks associated to dogs in peri-urban areas of Argentina. Comparative Immunology, Microbiology and Infectious Diseases, 53:40-44.

Cicuttin, G.L., De Salvo, M.N., Silva, D.A., Brito, M., Nava, S. (2017) Ehrlichia canis (Rickettsiales: Anaplasmataceae) en garrapatas Rhipicephalus sanguineus sensu lato del linaje templado (Acari: Ixodidae), provincia de Buenos Aires, Argentina. FAVE. Sección Ciencias Veterinarias, 16:93-96.

Cicuttin, G.L., Tarragona, E.L., De Salvo, M.N., Mangold, A.J., Nava, S. (2015). Infection with Ehrlichia canis and Anaplasma platys (Rickettsiales: Anaplasmataceae) in two lineages of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) from Argentina. Ticks and Tick Borne Diseases, 6 (6):724-729.

Dantas-Torres, F., Chomel, B.B., Otranto, D. (2012). Ticks and tick-borne diseases: a One Health perspective. Trends in Parasitology, 28:437-446.

Eiras, D., Craviotto, M.B., Vezzani, D., Eyal, O., Baneth, G. (2013). First description of natural Ehrlichia canis and Anaplasma platys infections in dogs from Argentina. Comparative Immunology, Microbiology and Infectious Dieases, 36:169-173.

Harrus, S., Waner, T., Aizenberg, I., Foley, J.E., Poland, A.M., Bark, H. (1998). Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. Journal of Clinical Microbiology, 36:73-76.

Harrus, S., Alleman, A.R., Bark, H., Mahan, S.M., Waner, T. (2002). Comparison of three enzyme-linked immunosorbant assays with the indirect immunofluorescent antibody test for the diagnosis of canine infection with Ehrlichia canis. Veterinary Microbiology, 86(4):361-368.

Harrus, S., Waner, T. (2011). Diagnosis of canine monocytotropic ehrlichiosis (Ehrlichia canis): an overview. Veterinary Journal, 187(3):292-296.

Harrus S., Waner, T., Neer, T.M. (2012). Ehrlichia canis infection. In: Greene C.E. (Ed.), Infectious Diseases of the Dog and Cat. Missouri, Elsevier. 227-238.

Maggi, R.G., Birkenheuer, A.J., Hegarty, B.C., Bradley, J.M., Levy, M.G., Breitschwerdt, E. (2014). Comparison of serological and molecular panels for diagnosis of vector-borne diseases in dogs. Parasites & Vectors, 7(1):127.

Mansilla Fernández, S.L., Sotelo, A.A., Delgado, M.B., Cainzos, R.P., Deluca, G.D., Koszcinczuk, P. (2019). Ehrlichiosis canina en el nordeste argentino: confirmación diagnóstica por PCR. Resultados preliminares. Memorias XV Congreso Argentino de Microbiología, V Congreso Argentino de Microbiología de Alimentos, V Congreso Latinoamericano de Microbiología de Medicamentos y Cosméticos, XIV Congreso Argentino de Microbiología General, Asociación Argentina de Microbiología. Bs. As., Argentina, p.93.

Martin, P., De Salvo M.N., Cicuttin, G.L., Arauz, M.S. (2019). Canine monocytic ehrlichiosis in Buenos Aires, Argentina: comparison of serological and molecular assays. Pesquisa Veterinária Brasileira, 39(8):649-654.

Mascarelli, P.E., Tártara, G.P., Pereyra, N.B., Maggi, R.G. (2016). Detection of Mycoplasma haemocanis, Mycoplasma haematoparvum, Mycoplasma suis and other vector-borne pathogens in dogs from Córdoba and Santa Fé, Argentina. Parasites & Vectors, 15; 9(1):642.

Mera y Sierra R., Neira, G. (2014) Ocurrencia de Ehrlichia canis en caninos de la provincia de Mendoza. III Congreso Panamericano de Zoonosis and VIII Congreso Argentino de Zoonosis, La Plata, Argentina.

Moraes-Filho, J., Marcili, A., Nieri-Bastos, F., Richtzenhain, L.J., Labruna, M.B., (2011). Genetic analysis of ticks belonging to the Rhipicephalus sanguineus group in Latin America. Acta Tropica, 117 (1):51-55.

Moraes-Filho, J., Soares, J.B., da Silva Krawczak, F., Lado, P., Labruna, M.B., (2013). Estudio de la competencia vectorial de Ehrlichia canis por cuatro poblaciones de Rhipicephalus sanguineus. Acta Médica Costarricense, 55, 93.

Murphy, G.L., Ewing, S.A., Whitworth, L.C., Fox, J.C., Kocan, A.A. (1998). A molecular and serologic survey of Ehrlichia canis, E. chaffeensis and E. ewingii in dogs and ticks from Oklahoma. Veterinary Parasitology, 27;79(4):325-339.

Nava, S., Mastropaolo, M., Venzal, J.M., Mangold, A.J., Guglielmone, A.A., (2012). Mitochondrial DNA analysis of Rhipicephalus sanguineus sensu lato (Acari:Ixodidae) in the Southern Cone of South America. Veterinary Parasitology, 190, 547–555.

Neer, T.M., Breitschwerdt, E.B., Greene, R.T., Lappin, M.R. (2002). Consensus statement on ehrlichial disease of small animals from the infectious disease study group of the American College of Veterinary Internal Medicine. Journal of Veterinary Internal Medicine, 16:309-315.

Otranto, D., Testini, G., Dantas-Torres, F., Latrofa, M.S., Diniz, P.P., Caprariis, D., Lia, R.P., Mencke, N., Stanneck, D., Capelli, G., Breitschwerdt, E.B. (2010). Diagnosis of canine vector-borne diseases in young dogs: a longitudinal study. Journal of Clinical Microbiology, 48 (9):3316-3324.

Palomeque, S.M., Eiras, D.F., Vazquez, M.V. (2019). Hepatozoonosis canina: hallazgos hematológicos y bioquímicos en perros de la ciudad de Córdoba, Argentina. Universidad Nacional de la Plata, Facultad de Ciencias Veterinarias, Trabajo final Especialización en Diagnóstico Veterinario de Laboratorio, 33 pp.

Sánchez, R., Bazzano, V., Felix, M., Armúa-Fernández, M., Venzal, J. (2020). Ehrlichiosis monocítica canina en la provincia de Entre Ríos, Argentina: confirmación molecular de casos en la ciudad de Concordia. FAVE Sección Ciencias Veterinarias, 19 (1):16-22.

Shaw, S.E., Day, M.J., Birtles, R.J., Breitschwerdt, E.B. (2001). Tick-borne infectious diseases of dogs. Review. Trends in Parasitology, 17 (2):74-80.

Tarragona, E.L., Flores, F.S., Herrera, C.L., Dalinger, M., Aguirre, N., Monje, L.D., Nava, S. (2019). Primer reporte de un caso de ehrlichiosis monocítica canina en la provincia de Santa Fe, Argentina. FAVE Sección Ciencias Veterinarias, 18: 49-54.

Waner, T., Harrus, S., Jongejan, F., Bark, H., Keysary, A., Cornelissen, A.W. (2001). Significance of serological testing for ehrlichial diseases in dogs with special emphasis on the diagnosis of canine monocytic ehrlichiosis caused by Ehrlichia canis. Veterinary Parasitology, 95 (1):1-15.

Published

2023-02-01

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How to Cite

Corina Guendulain, Pablo Tamiozzo, Aníbal Bessone, Marina Caffaratti, Fernanda Giménez, Sebastián Cabello, & Mariana Bonacci. (2023). Molecular detection of Ehrlichia canis in dogs from different locations of the province of Córdoba, Argentina. Ab Intus, (7). Retrieved from http://www.ayv.unrc.edu.ar/ojs/index.php/Ab_Intus/article/view/31

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Comunicaciones breves