Standardization of bovine tuberculin in cattle

Authors

  • Amelia Bernardelli Ceva Salud Animal https://orcid.org/0009-0002-7621-3419
  • Camila Miotti Instituto de Investigación de la Cadena Láctea (INTA – CONICET), Argentina https://orcid.org/0000-0002-7700-3440
  • Luciano Villosio Estudio Veterinario A.V.I.S., Argentina
  • Tomás Martino
  • Florencia Tonini Estudio Veterinario A.V.I.S., Argentina
  • Pablo Buraschi Estudio Veterinario A.V.I.S., Argentina
  • Pablo Costamagna Estudio Veterinario A.V.I.S., Argentina
  • Andrés Baima Estudio Veterinario A.V.I.S., Argentina
  • Augusto Briggiler Estudio Veterinario A.V.I.S., Argentina
  • Javier Rui Estudio Veterinario A.V.I.S., Argentina
  • Carlos Martino Estudio Veterinario A.V.I.S., Argentina
  • Victoria Reynalds Ceva Salud Animal, Argentina
  • Fernando Martino Estudio Veterinario A.V.I.S., Argentina
  • Marcelo Signorini Instituto de Investigación de la Cadena Láctea (INTA – CONICET), Argentina https://orcid.org/0000-0001-6537-8782

Abstract

ABSTRACT

Bovine tuberculosis is a cronic infectious bacterial disease produced by  Mycobacterium bovis .Infection in a live animal is diagnosed by hypersensitivity reaction delayed by intradermal immunization of bovine PPD.Laboratory control of  the biological potency of Bovine PPD is carried out on nine guinea pigs sensitized with in the homologous strain.World Helath Organization (WHO) Tecnical Report Series Protocol N°384 recommends complementing these controls with tests performed on sick bovines.The objective of this study was to evaluate the biological potency and stability of PPD applied in bovines with naturally acquired tuberculosis under the conditions  in which the reagent will be used .Three  series of Bovine PPD tuberculinr from Ceva  Animal Health were analyzed in comparison with the reference standar ( using a Latin square design ) for nine naturally tuberculous bovines and on eight inoculations sites on both sides of the animal neck.They were applied in commercial doses and 1/5 dilutions,registering the skin thicknesss reactions 72 h pos-inoculation.The reactions produced by the Ceva Bovine PPD series were higher than those produced by the standar PPD. The tuberculin potency and stability values were maintained for 13,14 and 15 months after the approval,thus concluding the stability and  potency of the formulation and quality of this commercial diagnostic reagent  for animal tuberculosis.

Key words : Quality control,tuberculosis bovine,tuberculin PPD,potency,stability,Argentina.

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Published

2023-12-28 — Updated on 2024-01-05

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How to Cite

Bernardelli, A., Miotti, C. ., Villosio, L. ., Martino, T. ., Tonini, F., Buraschi, P., Costamagna, P. ., Baima, A. ., Briggiler, A. ., Rui, J. ., Martino, C. ., Reynalds, V. ., Martino, F. ., & Signorini, M. . (2024). Standardization of bovine tuberculin in cattle. Ab Intus, 6(12). Retrieved from http://www.ayv.unrc.edu.ar/ojs/index.php/Ab_Intus/article/view/114 (Original work published December 28, 2023)

Issue

Vol. 6 No. 12 (2023): Ab Intus 12

Section

Comunicaciones breves

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