Efecto del extracto hexánico de Achyrocline satureioides sobre el crecimiento de Paenibacillus larvae, patógeno de Apis mellifera

Autores/as

  • María Fernanda Paletti Rovey Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto.
  • Diana Carolina Pimentel Betancourt Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto.
  • María de la Paz Moliné Centro de Investigación en Abejas Sociales (CIAS), Instituto de Investigaciones en Producción Sanidad y Ambiente, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata. CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas).
  • María de las Mercedes Oliva Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto. CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas).

Resumen

La salud de Apis mellifera es un tema importante debido al rol ecológico y económico que cumplen las abejas. Paenibacillus larvae, un bacilo formador de esporas, es el principal patógeno bacteriano de las larvas de abejas melíferas y es el agente causal de Loque Americana. Esta enfermedad provoca pérdidas económicas considerables y su control es un desafío para los apicultores de todo el mundo. Por lo tanto, es necesario encontrar tratamientos efectivos y, en este sentido, se están estudiando productos naturales derivados de plantas medicinales. En este trabajo se determinó la concentración inhibitoria mínima (CIM) y la concentración bactericida mínima (CBM) del extracto hexánico de Achyrocline satureioides, obteniendo valores de 0,30 μg/ml y 1,17 μg/ml, respectivamente. Se determinaron los parámetros de crecimiento de P. larvae 9 (ERIC I) y luego se evaluó el efecto de diferentes concentraciones del extracto hexánico (sub-inhibitoria, inhibitoria, sub-bactericida y bactericida) sobre el crecimiento bacteriano. El extracto hexánico mostró un buen efecto inhibitorio, independientemente de las concentraciones evaluadas. El crecimiento bacteriano fue afectado significativamente a concentraciones sub-bactericidas y bactericidas. Nuestros resultados demostraron la efectividad del extracto hexánico de A. satureioides sobre P. larvae, constituyendo una alternativa prometedora para el control de Loque Americana.

DOI: https://doi.org/10.5281/zenodo.7484788

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12/30/2022

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Paletti Rovey, M. F., Pimentel Betancourt, D. C., Moliné, M. de la P., & Oliva, M. de las M. (2022). Efecto del extracto hexánico de Achyrocline satureioides sobre el crecimiento de Paenibacillus larvae, patógeno de Apis mellifera. Ab Intus, (10), 33–49. Recuperado a partir de http://www.ayv.unrc.edu.ar/ojs/index.php/Ab_Intus/article/view/43

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