Effect of the hexanic extract of Achyrocline satureioides on Paenibacillus larvae growth, pathogen of Apis mellifera
Abstract
The health of Apis mellifera hives is an important issue due to the ecological and economic role that bees play. Paenibacillus larvae, a spore-forming bacillus, is the main bacterial pathogen of bees larvae and the causative agent of American foulbrood. This disease causes considerable economic losses and its control is a challenge for beekeepers around the world. Therefore, there is a need to find effective treatments and natural products derived from medicinal plants are being studied. In the present work, the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the hexanic extract of Achyrocline satureioides were determined, obtaining values of 0.30 μg/ml and 1.17 μg/ml, respectively. The growth parameters of P. larvae 9 (ERIC I) were determined, and then the effect of different concentrations of the hexanic extract (sub-inhibitory, inhibitory, sub-bactericidal and bactericidal) on the bacterial growth was evaluated. The hexanic extract showed a good inhibitory effect regardless of the concentrations tested. Bacterial growth was significantly affected at sub-bactericidal and bactericidal concentrations. Our results demonstrated the effectiveness of the hexanic extract of A. satureioides on P. larvae growth, constituting a promising alternative for American foulbrood control.
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