Bombesin determination in pig’s stomach with gastritis diagnose caused by Helicobacter spp.
Abstract
The objective of this study was to analyze the relationship between Helicobacter
spp and the functionality of bombesin-producing cells in gastric biopsies of pigs.
The presence of Helicobacter spp was determined in gastric samples from the
antro-pyloric region of mixed-breed pigs. They were colored with Hematoxylin/
Eosin for the diagnosis of gastritis, finding a higher proportion of chronic
than acute lesions. The presence of Helicobacter was carried out by means
of Gi-emsa and Warthin-Starry staining, determining the presence of, at least,
two species colonizing the stomach of the pig: one of larger size, intensely spiral
He-licobacter heilmannii); and another of smaller size, in the form of “s” italic
(He-licobacter pylori). Helicobacter spp was localized in the epithelium, fossa
and gastric glands by immunohistochemistry. The detection of bombesin producing
cells was performed by immunohistochemical techniques and its percentage
was determined in samples with acute and chronic gastritis, Helicobacter (+) /
(-). These cells were in the glandular epithelium, underlying connective tissue
and muscle tissue. Statistically significant difference was observed between the
groups: Chronic Gastritis Helicobacter (+) / Acute Gastritis Helicobacter (+) (p
= 0.04); Chronic Gastritis Helicobacter (+) / Normal Mucosa (p = 0.02) and in
Chronic Gastritis Helicobacter (-) / Normal Mucosa (p = 0.005). The group with
the highest number of bombesin positive cells was that of Gastritis Acute Heli-
cobacter (+), whose cell morphology was closed type.
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References
Atherton, J.C; Blaser M.J. (2009). Coadaptation of Helicobacter pylori and humans: ancient history, modern implications. J Clin Invest; 119:2475-2487.
Barbosa, A.; Silva, J.; Nogueira, A.; Paulino, E. y Miranda, C. (1995). Higher incidence of Gastrospirillum sp in swine with gastric ulcer of the pars oesophagea. Vet Pathol; 32:134-139.
Baele, M.; Decostere, A.; Vandamme, P.; Peelen, L.; Hellemans, A.; Mast, J.; Chiers, K.; Ducatelle, R. y Haesebrouck, F.(2008). Isolation and characterization of Helicobacter suis sp. from pig stomachs. Intern Journ of System and Evol Microb. 58(6): 1350-1358.
Bento-Miranda, M. y Figueiredo, C. (2014). Helicobacter heilmannii sensu lato: An overview of the infection in humans. World Journ of Gastroent. : 20(47): 17779-17787.
De Witte, C.; Devriendt, B.; Flahou, B.; Bosschem, I.; Ducatelle, R.; Smet, A. y Haesebrouck, F. (2017). Helicobacter suis induces changes in gastric inflammation and acid secretion markers in pigs of different ages. Vet Res 48: 34.
Di Rienzo, J.; Casanoves, F.; Balzarini, M.; Gonzalez, L.; Tablada, M. y Robledo, C.(2011). InfoStat versión 2011. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. URL http://www.infostat.com.ar
Fox, J.; Dangler, C.; Sager, W.; Borkowski, R. y Gliato, J.(1997).Helicobacter mustelae associated gastric adenocarcinoma in ferrets (Mustelae putorius furo).Vet Pathol; 34:125-129.
Gimeno, E.; Massone, A. Y Portiansky, E. (1997). Introducción a las Técnicas de
inmunohistoquímica y Aplicaciones en Patología Veterinaria. Manual del Octavo Curso Internacional de Posgrado en Técnicas de Inmunohistoquímica,
Lectínhistoquímica y Microscopía Electrónica. Instituto de Patología “Dr. Bernardo Epstein” y Servicio Central de Microscopía Electrónica, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata. La Plata ; p.49-77.
Gómez, G., Leonardo, F.; Orozco, P.; Salas, S. y Sergio, A. (2006). Helicobacteriosis canina y felina. Vet Méx, vol. 37/ 1: 97-116
Goodwin, C. y Armstrong, J.(1989). Aspect microbiological of Helicobacter pylori (Campylobacter pylori) .Eur. J. Clin. Microb. Infec.Dis, 9 (1): 1-13.
Graham, DV.; Opekun, A.; Lew, GM.; Klein, P. y WLash, JH.(1991). Helicobacter pylori associated exaggerated gastrin release in duodenal ulcer patients. The effect of bombesin and urea ingestion. Gastroent 100: 1571-5.
Gutierrez, O. (1988). Péptidos reguladores del sistema digestivo. Acta Med Colomb Vol 13 N°4: 254-258
Haesebrouck, F., Pasmans, F., Flahou, B., Smet, A., Vandamme, P., y Ducatelle, R. (2011). Non-Helicobacter pylori Helicobacter species in the human gastric mucosa: a proposal to introduce the terms H. heilmannii sensu lato and sensu stricto. HELICOB 16 (4): 339–340.
Hobsley, M.; Tovey, F. y Holton, J. (2008). Controveries in the Helicobacter pylori/duodenal ulcer story. R. Soc.Trop Med Hyg.102(12):1171-1175.
Liang, J.; Ducatelle, R.; Pasman, F.; Annemieke, S.; Haesebrouck, F. y Flahou, B. (2013). Sequence typing of porcine and human gastric pathogen Helicobacter suis. Int J Syst and Evol Microbiol, 51(3): 920-936.
Levi, S.; Beardshall, K.; Haddad, G.; Playford, R.; Ghosh, P. y Calam, J. (1989). Campylobacter pylori and duodenal ulcers: thegastrin link. Lancet; i: 1167-8.
Mac Loughlin, V. (2014). Relación entre Helicobacter spp. y células productoras de Gastrina. Tesis de Doctorado. Universidad Nacional de Río Cuarto, Río Cuarto, Argentina. 104 p.
Mac Loughlin, V.; Merkis, C.; Dauria; P.; Cristofolini, A.; Bonino, F.; Sagripanti, G.; Sanchis, E.; Ritta, L. y Savino, F. (2015). Determinación de células G en estómago de cerdo con Helicobacter spp. Rev. CVPBA, 63:76-77.
Mac Loughlin, V.; Dauría, P.; Van Deer Veen, P.; Bonino, F.; Sagripanti, G.; Martínez, R.; Navarro, O.; Giménez, S.; Sona, L.; Ritta L. y Hernández, A. (2016). Inmunoexpresión de células productoras de bombesina en estómago de cerdos con diagnóstico de gastritis crónica provocada por Helicobacter spp. 3° Congreso Virtual de Ciencias Morfológicas.
Makinde, M. y Obwolo, M.(1990). Abattoir survey of gastric ulcer in pigs in Zimbabwe. Zimb Vet J; 21:116-123.
Mc Coll, K.; El-Omar, R. y Guillen, D. (1997). The role of Helicobacter pylori in the pathophysiology of duodenal ulcer and gastrin cancer. Semin Gastrointest disease, 8:142-155.
Morales, A. y Bermudez, V. (2008). Modelos Animales para el estudio de la infección por el género Helicobacter en Humanos. Rev. Soc. Med. Hosp. Emerg. Perez de Leon ; 39(1):30-33.
Moreno, R. (2005). La bacteria que destronó al stress. Diario El país. España.
Odriozola, V.(2011). Helicobacter gastricos en caninos y felinos. Asociación argentina de medicina Felina.URL: http:/www.aamefe.org/helicobacter.htm.
Pacheco Piñol Jiménez, F. y Paniagua Estévez, M. (2006). Neuropéptidos y Helicobacter pylori en la gastritis crónica- Rev cubana med v.45 n.3.
Paz, D. (2000). Técnicas Inmunocitoquímicas. En: Guía de Curso de Posgrado. Departamento de Ciencias Biológicas. Facultad de Ciencias Exactas. Universidad Nacional de Buenos Aires. Buenos Aires , Cap.5:21-23.
Piqueras Ruiz, L. (2004). El control de la secreción ácida gástrica en el ratón: cambios asociados a la gastritis o a la falta del receptor de tipo 2 de la somatostatina. Tesis de doctorado. Universidad de Valencia. España, 145 p.
Queiroz, D.; Rocha, G.; Mendes, E.; De Moura, S. y Rocha, A. (1996). Association between Helicobacter and gastric ulcer disease of the pars oesophagea in swine. Gastroent; 111:19-27. 103.
Rodriguez, B.; Aranzazu, D. Y Ortiz, L. (2009). Association of gastric ulcer and Helicobacter spp in pigs in Antioquia, Colombia. Rev. Colom Cien. Pecua, 22:54-60.
Smet, A.; Flahou, B.; D’Herde, K.; Vandamme, P.; Cleenwerck, I.; Ducatelle, R.; Pasmans, F. y Haesebrouck, F. (2012). Helicobacter helimannii sp. nov., isolated from feline gastric mucosa. Internat Journ System Evol Microb 62: 299-306
Tytgort, G. (1994). Long-term consecuenses of Helicobacter pylori erradication. Scand J Gastroenterol, 205: 38-44.
Valdivia Roldán, M. (2011).Gastritis y gastropatías. Rev Gastroenterol. Perú; 31/1: 38-48.
Vikram, K.; Ananthakrishnan, N. y Tovey, F. 2013. Is Helicobacter infection the primary cause of duodenal ulceration or secondary factor? a review of the evidence. Gastroent Res. Pract, 425840.
Vizzio, P. (2000). Técnicas Inmunocitoquímicas. En: Guía de Curso Posgrado.
Departamento de Ciencias Biológicas. Facultad de Ciencias Exactas.
Universidad Nacional de Buenos Aires. Buenos Aires:Cap.5:21-23.
Warren, J. (1997). The discovery and pathology of Helicobacter pylori. In Xth int. workshop on gastroduodenal pathology and Helicobacter pylori: the logic of eradication. 7-13.
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