Mixed probiotic/anti-mycotoxin additive (Saccharomyces cerevisiae RC016 and Lactobacillus rhamnosus RC007) supplementation of AFB1-contaminated feed influences broiler chickens productive parameters, biochemistry and liver/intestine histopathology
Keywords:
broiler chickens, probiotic, antibiotic, aflatoxin B1, growth performance.Abstract
The objective of the present work was to study the influence of dietary supplementation of a probiotic and anti-mycotoxin mixed additive (MA, Saccharomyces cerevisiae RC016 and Lactobacillus rhamnosus RC007) and their interaction on the performance and health (biochemistry and livers/intestines histopathology) of broiler chickens fed aflatoxin B1 (AFB1) contaminated diets. A total of 60 one-day-old Cobb broilers were randomly allocated into four treatment groups with three replicates of 5 birds each for a five-week d feeding experiment. The dietary experimental of each treatment (T) were formulated as follows: T1, a commercial diet (CD); T2, CD + AFB1 (506.14 ± 22.1 ng/kg); T3, CD + 0.1% MA, the ratio of S. cerevisiae RC016 (1 x 107 cells/g) to L. rhamnosus RC007 (1 x 108 cells/g) was 1:1; T4, CD + AFB1 (506.14 ± 22.1 ng/kg) + 0.1% MA. The MA improved (p<0.01) production parameters (weight gain, conversion rate, and carcass yield) and reduced (p < 0.01) the toxic effect of AFB1 on the relative weight of the livers. In addition, the macro and microscopic alterations of livers and the possible intestinal injury related to histological damage in the presence of mycotoxin were reduced. The use of probiotic MA based on S. cerevisiae RC016 and L. rhamnosus RC007 in animal feed provides greater protection against mycotoxin contamination and is safe for use as a supplement in animal feed, exercising benefit effects that improve animal health and productivity. This is of great importance at the economic level for the avian production system.
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