Injection of prostaglandin F2 alpha (PGF2α) into the mare’s preovulatory follicle
Abstract
Prostaglandins (PGE2 and PGF2α) play an essential role in the ovulation process in mammals. The objective of this experiment was to determine whether intrafolicular injection with prostaglandin F2 alpha (PGF2α) administered in the pre-ovulation period induced normal ovulation and corpus luteum formation, it was later confirmed with the presence of an embryonic vesicle. Six mixedbreed mares were used in two estrous cycles each, with a crossover design. The ultrasonographic examination of the reproductive tract was performed every 24 hours from day 15 of the cycle (Day 0 = day of ovulation) until the time of follicular puncture (Hour 0). The ultrasound guided puncture of the preovulatory follicle (diameter ≥ 35 mm and presence of moderate uterine edema) was performed through the flank, injecting 750 μg of PGF2α diluted in 0.5 ml of sterile water (treated group n = 6) or placebo (0.5 ml of injection water) (control group n = 6). Prior to puncture, the mares were inseminated with fresh semen.
Ultrasound images were obtained every 12 h of the punctured follicle and blood samples were taken every 48 h from the jugular vein to determine plasma progesterone levels. Five of the six mares (83%) of the treated group had ovulation failures and only one of the mares ovulated but no pregnancy was obtained. According to these results, intrafolicular injection with PGF2α at the concentration used would not allow normal ovulation and contrary to expectations would induce the formation of anovulatory hemorrhagic follicles. In addition, a delay in progesterone rise was observed in the only mare that ovulated.
Downloads
References
Adams, J., Liu, Z., Ren, Y. A., Wun, W. S., Zhou, W., Kenigsberg, S., Librach, C., Valdes, C., Gibbons, W., Richards, J. (2016). Enhanced inflammatory transcriptome in the granulosa cells of women with polycystic ovarian syndrome. The Journal of Clinical Endocrinology & Metabolism. 101 (9): 3459-3468.
Aguilar, J., Castañeira, C., Alonso, C., Flores, A., Marín, J., Cuervo-Arango, J., Martinez-Boví, R., Mougelar, H., Losinno, L. (2018). Intrafollicular injection of prostaglandins in the preovulatory follicle of the mare. Journal of Equine Veterinary Science. 66: 183.
Aguilar, J., Cuervo-Arango, J., Losinno, L. (2015). Folículos Hemorrágicos Anovulatorios en la yegua. Nuevos conceptos sobre un viejo problema en clínica reproductiva. Biotecnologías Reproductivas en Equinos I. FAVE Eds (ISBN 978-987-45862-1-6). 173-187.
Conti, M., Hsieh, M., Park, J. Y., Su, Y. Q. (2006). Role of the epidermal growth factor network in ovarian follicles. Molecular Endocrinology. 20 (4): 715-723.
Couse, J. F., Yates, M. M., Deroo, B. J., Korach, K. S. (2005). Estrogen receptor-β is critical to granulosa cell differentiation and the ovulatory response to gonadotropins. Endocrinology. 146 (8): 3247-3262.
Cuervo-Arango, J. & Domingo-Ortiz, R. (2011). Systemic treatment with high dose of flunixin-meglumine is able to block ovulation in mares by inducing hemorrhage and luteinisation of follicles. Theriogenology. 75: 707–714.
Cuervo-Arango, J. & Newcombe, J.R. (2010). Risk factors for the development of haemorrhagic anovulatory follicles in the mare. Reproduction in Domestic Animals. 45: 473-480.
Cuervo-Arango, J. & Newcombe, J.R. (2012). Ultrasound characteristics of experimentally induced luteinized unruptured follicles (LUF) and naturally occurring hemorrhagic anovulatory follicles (HAF) in the mare. Theriogenology. 77: 514-524.
Daels, P. F. & Hughes, J. P. (1993). The normal estrous cycle. In McKinnon, A. O. and Voss J. L., editors: Equine Reproduction, 1st Edition. Philadelphia: Lea and Febiger. 121-132.
Edelman, A.B, Jensen, J.T., Doom, C., Hannebold, J.D. (2013). Impact of the prostaglandin synthase-2 inhibitor celecoxib on ovulation and luteal events in women. Contraception. 87: 352 – 357.
Gastal, E. L. (2011). Ultrasonographic morphologic of the preovulatory follicle. In Mckinnon, A. O.; Squires E. L.; Vaala, W. E.; Varner,D. D., editors: Equine Reproduction, 2nd Edition. United Kingdom: Wiley-Blackwell. 2032-2054.
Gaytán, F., Tarradas, E., Bellido, C., Morales, C., Sánchez-Criado, J. E. (2002). Prostaglandin E1 inhibits abnormal follicle rupture and restores ovulation in indomethacin-treated rats. Biology of Reproduction. 67 (4):1140-1147.
Gérard, N. & Robin, E. (2019). Cellular and molecular mechanisms of the preovulatory follicle differenciation and ovulation: What do we know in the mare relative to other species. Theriogenology. 160: 163-179.
Ginther, O. J., Gastal, E. L., Gastal, M. O., Beg, M. A. (2008). Dynamics of the equine preovulatory follicle and periovulatory hormones: what’s new? Journal of Equine Veterinary Science. 28 (8): 454-460.
Ginther, O.J., Beg, M.A., Gastal, E.L., Gastal, M.O., Cooper, D.A. (2009). Treatment with human chorionic gonadotropin (hCG) for ovulation induction is associated with an immediate 17β-decrease and a more rapid LH increase in mares. Animal Reproduction Science. 114: 311-317.
Ginther, O.J., Gastal, E.L., Gastal, M.O., Beg, M.A. (2007). Incidence, endocrinology, vascularity, and morphology of hemorrhagic anovulatory follicles in mares. Journal of Equine Veterinary Science. 27: 130-139.
Hsieh, M., Lee, D., Panigone, S., Horner, K., Chen, R., Theologis, A., Lee, D.C., Threadgill, D.W., Conti, M. (2007). Luteinizing hormone-dependent activation of the epidermal growth factor network is essential for ovulation. Molecular and cellular biology. 27 (5): 1914-1924.
Kim, J., Bagchi, I. C., Bagchi, M. K. (2009). Control of ovulation in mice by progesterone receptor-regulated gene networks. Molecular human reproduction. 15 (12): 821-828.
Larentis, G.R., Bastos, H.B.A., Camozzato, G.C., Santos, G.O., Mattos, R.C. (2018). Do therapeutic doses of phenilbutazone affect ovulation process in mare. Journal of Equine Veterinary Science. 66: 150-151.
Lefranc, A. C. & Allen, W. R. (2003). Incidence and morphology of anovulatory haemorrhagic follicles in the mare. Pferdeheilkunde. 19 (6): 611-612.
Li, Q., Jimenez-Krassel, F., Kobayashi, Y., Ireland, J.J., Smith, G.W. (2006). Effect of intrafollicular indomethacin injection on gonadotropin surge-induced expression of select extracellular matrix degrading enzymes and their inhibitors in bovine preovulatory follicles. Reproduction.131: 533-544.
Lim, H., Paria, B. C., Das, S. K., Dinchuk, J. E., Langenbach, R., Trzaskos, J. M., Dey, S. K. (1997). Multiple female reproductive failures in cyclooxygenase 2–deficient mice. Cell. 91 (2): 197-208.
Martínez-Boví, R. & Cuervo-Arango, J. (2016). Follicle Diameter and Systemic Hormone Interrelationships during Induction of Follicle Collapse with Intrafollicular Prostaglandin E2 and F2α in Mares. Reproduction in Domestic Animals. 51: 322–329
Martínez-Boví, R. & Cuervo-Arango, J. (2016). Intrafollicular treatment with prostaglandins PGE2 and PGF2α inhibits the formation of luteinized unrupture follicles and restores normal ovulation in mares treated with flunixin-meglumine. Equine Veterinary Journal. 48: 211 – 217
McCue, P. M. & Squires, E. L. (2002). Persistent anovulatory follicles in the mare. Theriogenology. 58 (2-4): 541-543.
Murdoch, W. J., Hansen, T.R., McPherson, L.A. (1993). A Review - Role of ecosanoids in vertebrate ovulation. Prostaglandins. 46: 85-115
Niringiyumukiza, J. D., Cai, H., Xiang, W. (2018). Prostaglandin E2 involvement in mammalian female fertility: ovulation, fertilization, embryo development and early implantation. Reproductive Biology and Endocrinology. 16: 43.
Paredes, P., Jiménez, C., Hernández, A. (2013). Corpus luteum diameter and serum progesterone level, during the estrous cycle in colombian criollo mares. Revista MVZ Córdoba. 18 (3): 3829-3835.
Peluffo, M.C., Stanley, J., Braeuer, N., Rotgeri, A., Fritzemeier, K.H., Fuhrmann, U., Buchmann B., Adevai, T., Murphy, M.J., Zelinski, M.B., Lindenthal, B., Hennebold, J.D., Stouffer, R.L. (2014). A prostaglandin E2 receptor antagonist prevents pregnancies during a preclinical contraceptive trial with female macaques. Human Reproduction. 29 (7): 1400 –1412.
Pérez, R. S., Jiménez L.M., Gómez Danés A.A., Casiano Ventura M. A., Quero Carrillo A.R., Pérez Rodríguez, P. (2016). Piroxicam and ovarian changes in adult goat. Nova Scientia. 8: 91-105.
Richards, J. S. (2018). Ovulation. Elsevier. 92-98
Robker, R.L., Russell, D.L., Yoshioka, S., Sharma, S.C., Lydon, J.P., O’Malley, B.W., Espey, L.L., Richards, J.S. (2000). Ovulation: a multi-gene, multistep process. Steroids. 65: 559-570.
Sirois, J & Doré, M. (1997). The Late Induction of Prostaglandin G/H Synthase-2 in Equine Preovulatory Follicles Supports Its Role as a Determinant of the Ovulatory Process. Endocrinology. 138: 4427-34.
Song, J.H, Curry, T.E., Brannstorm, M., LeMaire, W.J., Koods, R.D., Papkoff, H., Janson, P.O. (1987). Inhibition of follicle-stimulating hormone-induced ovulation by indomethacin in the perfused rat ovary. Biology Reproduction. 36: 536-42.
Townson, D. H. & Ginther, O. J. (1987). Duration and pattern of follicular evacuation during ovulation in the mare. Animal Reproduction Science. 15 (1-2): 131-138.
Townson, D. H., Pierson, R. A., Ginther, O. J. (1989). Characterization of plasma progesterone concentrations for two distinct luteal morphologies in mares. Theriogenology. 32 (2): 197-204.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Ab Intus
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
