Molecular diagnosis of Hepatozoon canis in dogs: clinical signs and hematological results
Abstract
Canine hepatozoonosis is a parasitic disease acquired by ingesting ticks infected with protozoa of the genus Hepatozoon (H); H. canis and H. americanum are the two species that can infect dogs, the first being the only one reported in our country so far. The clinical signs and hematological aspects of this infection depend on the species involved and factors such as the immunological status of the animal and the parasite load. The objective of the study was to confirm the presence and determine the species of Hepatozoon by PCR in the blood of dogs diagnosed through the microscopic visualization of gamontes of Hepatozoon spp. and describe the clinical presentation and hematological results. The PCR technique was performed on 64 blood samples from dogs treated at the FAV School Hospital in which gamontes of Hepatozoon spp. in neutrophils and monocytes were found to the microscopic observation of the smear. A 96.9% of the blood samples were positive for the presence of Hepatozoon and the species found in all cases was H. canis. Clinical signs and characteristic hematological findings were increased temperature, pale mucous membranes, lymph node enlargement, ocular discharge, normochromic normocytic anemia and leukocyte values within the reference range. The importance of this study lies in the fact that the species causing canine hepatozoonosis has not been reported in our province.
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